No additional libraries are necessary.

Primer3Plus is not an application per se but rather a web interface to interact with Primer3. There is no compilation to be performed but you need to make sure that it is available to a local web browser using these instructions:

• The archive with the compiled tools is downloaded from the web in /home/bioubuntu, decompressed and the resulting folder is moved over into /opt/bio/sources.

• This folder should belong to root ans its permissions should be set to 755.

% sudo chown -R root:root /opt/bio/sources/primer3plus

• ONce you are in /opt/bio/sources/primer3plus, you have to copy its content to /opt/bio/docs/cgi-bin:

% sudo cp -r ./* /opt/bio/docs/cgi-bin

• The user that runs the Apache web server has to own /opt/bio/docs/cgi-bin:

% sudo chown -R www-data:www-data /opt/bio/docs/cgi-bin

• You need to modify the settings.pm file:

Before:

"PRIMER_BIN" =>  "./primer3_core",   # for Linux

After:

"PRIMER_BIN" =>  "/usr/local/bin/primer3_core",   # for Linux

• The web page should now be available using this URL: http://localhost/cgi-bin/primer3plus.cgi. Note: this will only work in an Impilo server…

• I added the following item into the Impilo > General purpose Tools menu.

% sudo xfce4-menueditor /etc/xdg/xfce4/desktop/menu.xml

• The following three items need to be added :
  • Item: Launcher
  • Name: Primer3Plus
  • Command: firefox http://localhost/cgi-bin/primer3plus.cgi