You will need the following libraries to compile and run the applications found in the SeqTools package (which now include Dotter):

• libgtk2.0-dev
• libcurl4-openssl-dev

% sudo apt-get install libgtk2.0-dev libcurl4-openssl-dev

Here is the procedure to build and install Dotter in Impilo:

• You need to download the source code from the Sanger Institute:

% wget ftp://ftp.sanger.ac.uk/pub4/resources/software/seqtools/PRODUCTION/seqtools-4.4.1.tar.gz

• You then need to decompress this archive and move it under /opt/bio/sources:

% tar -zxvf seqtools-4.4.1.tar.gz
% sudo seqtools-4.4.1 /opt/bio/sources

• In /opt/bio/sources, make sure that the seqtools-4.4.1 folder belongs to root and that the write permissions for Group and Other are removed:

% sudo chown -R root:root /opt/bio/sources/seqtools-4.4.1
% cd /opt/bio/sources/
% sudo chmod -R go-w seqtools-4.4.1

• Once you are inside seqtools-4.4.1, everything else is simple:

% cd seqtools-4.4.1
% sudo ./configure && sudo make && sudo make install && sudo make clean

• Although these applications are interactives and using a graphical user interface, you can only use via the command line interface because they need sequences as inputs to start.