====== Installation of Primer3Plus 1.0.0 ======

===== Additional Libraries =====

No additional libraries are necessary.

===== Procedure =====

Primer3Plus is not an application per se but rather a web interface to interact with [[primer3|Primer3]]. There is no compilation to be performed but you need to make sure that it is available to a local web browser using these instructions:

  * The archive with the compiled tools is downloaded from the web in ''/home/bioubuntu'', decompressed and the resulting folder is moved over into ''/opt/bio/sources''.

  * This folder should belong to ''root'' ans its permissions should be set to ''755''.

<code bash>
% sudo chown -R root:root /opt/bio/sources/primer3plus
</code>

  * ONce you are in ''/opt/bio/sources/primer3plus'', you have to copy its content to ''/opt/bio/docs/cgi-bin'':

<code bash>
% sudo cp -r ./* /opt/bio/docs/cgi-bin
</code>

  * The user that runs the Apache web server has to own ''/opt/bio/docs/cgi-bin'':

<code bash>
% sudo chown -R www-data:www-data /opt/bio/docs/cgi-bin
</code>

  * You need to modify the ''settings.pm'' file:

Before:

<code>
"PRIMER_BIN" =>  "./primer3_core",   # for Linux
</code>

After:

<code>
"PRIMER_BIN" =>  "/usr/local/bin/primer3_core",   # for Linux
</code>

  * The web page should now be available using this URL: [[http://localhost/cgi-bin/primer3plus.cgi|http://localhost/cgi-bin/primer3plus.cgi]]. Note: this will only work in an Impilo server...

  * I added the following item into the Impilo > General purpose Tools menu.  
<code>
% sudo xfce4-menueditor /etc/xdg/xfce4/desktop/menu.xml
</code>

  * The following three items need to be added :
    * Item: Launcher
    * Name: Primer3Plus
    * Command: ''firefox http://localhost/cgi-bin/primer3plus.cgi''