====== Installation instructions for SAMtools 0.1.8 ====== ===== Additional libraries ===== You need to install the following libraries: * ''libncurses-dev'' % sudo apt-get install -y libncurses-dev ===== Procedure ===== To install SAMtools from source code, you need to follow this recipe: * The archive with the source code was downloaded from the web site and decompressed in ''/home/bioubuntu'': % tar -jxvf samtools-0.1.8.tar.bz2 * **Warning!!** If you are compiling SAMtools on a 64-bit platform and you want to use it in building the Staden package, you will need to modify the ''Makefile'' file. You will need to edit the following line: # Before CFLAGS= -g -Wall -O2 #-m64 #-arch ppc # After CFLAGS= -g -Wall -O2 -m64 -fPIC #-arch ppc * The newly created folder is moved under ''/opt/bio/sources''. We need to make sure that it belongs to ''root'' and its permissions are set to ''755'': % sudo mv samtools-0.1.8 /opt/bio/sources % sudo chown -R root:root /opt/bio/sources/samtools-0.1.8 % sudo chmod 755 /opt/bio/sources/samtools-0.1.8 * Once you are in the folder, compilation is easily performed: % cd /opt/bio/sources/samtools-0.1.8 % sudo make * To make ''samtools'' available to all, you have to do this: % sudo ln -s /opt/bio/sources/samtools-0.1.8/samtools /usr/local/bin/samtools